
Genome-scale ordered mutant collections
For access to the genome-scale ordered mutant collections (GOMs) created by GutWorks, contact Dr. KC Huang
Bacteroides thetaiotaomicron VPI-5482
Arjes, H.A., Sun, J., Liu, H. et al. Construction and characterization of a genome-scale ordered mutant collection of Bacteroides thetaiotaomicron. BMC Biol 20, 285 (2022) https://doi.org/10.1186/s12915-022-01481-2
A member of the Bacteroidota, Bacteroides thetaiotaomicron VPI-5482 is a widely-used model system for the healthy human adult gut microbiota
The GOM is comprised of 34 96-well plates representing >2500 genes.
Download VPI-5482 ordered mutant collection map here
Bifidobacterium breve UCC2003
Shiver AL, Sun J, Culver R, et al. A mutant fitness compendium in Bifidobacteria reveals molecular determinants of colonization and host-microbe interactions. bioRxiv [Preprint], 2023.08.29.555234. (2023) https://doi.org/10.1101/2023.08.29.555234
A member of the Actinomycetota, Bifidobacterium breve UCC2003 has been used as a representative member of the healthy human infant gut microbiota
The GOM is comprised of 16 96-well plates representing 1462 genes.
Download the UCC2003 ordered mutant collection map here