Tools & Datasets
Experimental approaches, computational tools, and datasets
connected to Gutworks.
Browse thousands of genome-wide fitness experiments for diverse bacteria using the Fitness Browser, from the Deutschbauer lab, the Adam Arkin lab (Berkeley), and collaborators.
Single-cell phase-contrast imaging data
Bifidobacterium breve UCC2003 images can be accessed here
Single-cell phase-contrast microscopy images of the GOMs generated using SLIP by GutWorks
Microfluidic Devices for High-throughput Genetic Transformations
Garcia PA, Ge Z, Kelley LE, Holcomb SJ, Buie CR. High efficiency hydrodynamic bacterial electrotransformation. Lab Chip 17(3), 490–500 (2017) https://doi.org/10.1039/c6lc01309k.
The Buie lab have developed a transformative microfluidic device that enables the transformation of genetic material with 4x greater efficiency and up to 1000x higher throughput.
Huang PH, Chen S, Shiver AL, Culver RN, Huang KC, Buie CR. M-TUBE enables large-volume bacterial gene delivery using a high-throughput microfluidic electroporation platform. PLoS Biol 20(9), e3001727 (2022) https://doi.org/10.1371/journal.pbio.3001727
A low-cost, high-volume electroporation device developed by the Buie lab that accelerates the generation of large-scale mutant libraries